Advancements in fluorescent microscopy techniques now permit investigation of HIV-1 biology exploiting tools alternative to conventional molecular biology. Here we describe a novel, fluorescence-based method to visualize HIV-1 viral particles within intact nuclei of infected cells. This method allows investigating the localization of pre-integration complexes within the nuclear compartment with respect to the nuclear envelope and the chromatin territories.

Imaging HIV-1 nuclear pre-integration complexes.

GIACCA, MAURO
2014-01-01

Abstract

Advancements in fluorescent microscopy techniques now permit investigation of HIV-1 biology exploiting tools alternative to conventional molecular biology. Here we describe a novel, fluorescence-based method to visualize HIV-1 viral particles within intact nuclei of infected cells. This method allows investigating the localization of pre-integration complexes within the nuclear compartment with respect to the nuclear envelope and the chromatin territories.
2014
Pubblicato
http://dx.doi.org/10.1007/978-1-62703-670-2_5
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11368/2763697
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