Purpose: Latest improvements in the formulation of dentin bonding systems involved the addition of chlorhexidine (CHX) within the adhesive blends. Despite its possible antibacterial role, no information is available on CHX elution from the polymerized adhesive. Aim of this preliminary studywas to assess elution of CHX in water. The tested hypothesis was that no release of active material occurs over time. Methods and materials: Two universal adhesive systems were selected for the study: Peak Universal (Ultradent), and Scotchbond Universal (3M ESPE; control). Adhesive disks (1mm thick and 6mm in diameter) were prepared, fully cured using a LED lamp (VALO, Ultradent) for 3min and immersed in water for 72 h. Elution of CHX was monitored by an optimized and validated high-performance liquid chromatographic (HPLC) method. In details, the mobile phase was a binary mixture of a pH 3.0 triethylamine phosphate buffer and acetonitrile under gradient elution; the stationary phase was a Kinetex PFP column (5m, 150mm×4.6mm) and the detection wavelength was 254 nm. Results: The results of the study revealed that under the tested conditions, the selective analysis of chlorhexidine could be performed with limit of quantification (LOQ) of 0.1 ppm. Interestingly, no CHX was found in the eluates, despite the high sensitivity of the tested HPLC system. Conclusion: Further studies are needed to clarify the role of active compounds blended within the adhesive formulations.
HPLC analysis of adhesive eluates
MAZZONI, Annalisa;CHECCHI, Vittorio;
2014-01-01
Abstract
Purpose: Latest improvements in the formulation of dentin bonding systems involved the addition of chlorhexidine (CHX) within the adhesive blends. Despite its possible antibacterial role, no information is available on CHX elution from the polymerized adhesive. Aim of this preliminary studywas to assess elution of CHX in water. The tested hypothesis was that no release of active material occurs over time. Methods and materials: Two universal adhesive systems were selected for the study: Peak Universal (Ultradent), and Scotchbond Universal (3M ESPE; control). Adhesive disks (1mm thick and 6mm in diameter) were prepared, fully cured using a LED lamp (VALO, Ultradent) for 3min and immersed in water for 72 h. Elution of CHX was monitored by an optimized and validated high-performance liquid chromatographic (HPLC) method. In details, the mobile phase was a binary mixture of a pH 3.0 triethylamine phosphate buffer and acetonitrile under gradient elution; the stationary phase was a Kinetex PFP column (5m, 150mm×4.6mm) and the detection wavelength was 254 nm. Results: The results of the study revealed that under the tested conditions, the selective analysis of chlorhexidine could be performed with limit of quantification (LOQ) of 0.1 ppm. Interestingly, no CHX was found in the eluates, despite the high sensitivity of the tested HPLC system. Conclusion: Further studies are needed to clarify the role of active compounds blended within the adhesive formulations.Pubblicazioni consigliate
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