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EnglishBilirubin is an endogenous antioxidant in the human blood. While measurement of a total serum bilirubin, comprising of protein-bound (indirect bilirubin) and bilirubin glucuronide (direct bilirubin), is a routine clinical test, direct determination of free bilirubin represents an analytical challenge. Free bilirubin, which is the bioactive form, accounts for a minor fraction (<100 nM). Here we show the direct analysis of free bilirubin in human and animal blood serum samples using a state-of-the-art system comprised of newly developed high-performance liquid chromatography (HPLC) on reversed-phase (RP) C18 support coupled with thermal lens spectrometric detection (TLS), based on excitation wavelength of 457.9 nm using argon laser. The method excels in ultra-high sensitivity with limit of detection (LOD) and limit of quantitation (LOQ) of 90 pM and 250 pM, respectively. As a result, we present here the direct assay of free bilirubin in serum (around 10 nM), which can be employed as an assessment of antioxidative blood status.
| Titolo: | Antioxidative blood status assessment by the free bilirubin assay |
| Autori: | |
| Data di pubblicazione: | 2014 |
| Abstract: | Bilirubin is an endogenous antioxidant in the human blood. While measurement of a total serum bilirubin, comprising of protein-bound (indirect bilirubin) and bilirubin glucuronide (direct bilirubin), is a routine clinical test, direct determination of free bilirubin represents an analytical challenge. Free bilirubin, which is the bioactive form, accounts for a minor fraction (<100 nM). Here we show the direct analysis of free bilirubin in human and animal blood serum samples using a state-of-the-art system comprised of newly developed high-performance liquid chromatography (HPLC) on reversed-phase (RP) C18 support coupled with thermal lens spectrometric detection (TLS), based on excitation wavelength of 457.9 nm using argon laser. The method excels in ultra-high sensitivity with limit of detection (LOD) and limit of quantitation (LOQ) of 90 pM and 250 pM, respectively. As a result, we present here the direct assay of free bilirubin in serum (around 10 nM), which can be employed as an assessment of antioxidative blood status. |
| Handle: | http://hdl.handle.net/11368/2829667 |
| ISBN: | 978-88-8303-572-2 |
| URL: | http://hdl.handle.net/10077/10285 |
| Appare nelle tipologie: | 4.1 Contributo in Atti Convegno (Proceeding) |
