A series of fluorescent molecularly imprinted nanogels to detect irinotecan (CPT11) were prepared and characterized. A set of amino acids and napthalimide polymerisable derivatives allowed to obtain polymers as soluble fluorescent nanoparticles by high dilution imprinted synthesis. The direct detection of irinotecan in human plasma was obtained by fluorescence quenching of the naphtalimide-based imprinted materials. The plasma sample treated with acetonitrile allowed the detection of irinotecan in the 10nM – 30μM range. The LOD was 9.4 nM, with within-run variability 10% and day to day variability 13%.

Monitoring of Irinotecan in Human Plasma: Sensitive Fluorescent Nanogels by Molecular Imprinting

Berti, Federico
;
Bertoncin, Paolo;Forzato, Cristina;Posocco, Paola;Iacuzzi, Valentina;Pellizzoni, Elena;Tommasini, Martina
2019

Abstract

A series of fluorescent molecularly imprinted nanogels to detect irinotecan (CPT11) were prepared and characterized. A set of amino acids and napthalimide polymerisable derivatives allowed to obtain polymers as soluble fluorescent nanoparticles by high dilution imprinted synthesis. The direct detection of irinotecan in human plasma was obtained by fluorescence quenching of the naphtalimide-based imprinted materials. The plasma sample treated with acetonitrile allowed the detection of irinotecan in the 10nM – 30μM range. The LOD was 9.4 nM, with within-run variability 10% and day to day variability 13%.
Irinotecan dosages; Therapeutic drug monitoring; Imprinted polymeric materials; Biosensor experiments
File in questo prodotto:
File Dimensione Formato  
Tommasini Irinotecan_MT04.06.pdf

accesso aperto

Tipologia: Documento in Pre-print
Licenza: Creative commons
Dimensione 1.09 MB
Formato Adobe PDF
1.09 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2944780
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact