Assessment of DNA damage by Micellar Electrokinetic Chromathography

A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation



Titolo: Assessment of DNA damage by Micellar Electrokinetic Chromathography
Autori: 
FATTORINI, PAOLO
SORCABURU CIGLIERI, SOLANGE
mostra contributor esterni 
Data di pubblicazione: 2013
Serie: 
METHODS IN MOLECULAR BIOLOGY  
Abstract: A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation
Handle: http://hdl.handle.net/11368/2795325
Appare nelle tipologie:2.1 Contributo in Volume (Capitolo,Saggio)

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