A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation

Assessment of DNA damage by Micellar Electrokinetic Chromathography

FATTORINI, PAOLO;SORCABURU CIGLIERO, SOLANGE;
2013

Abstract

A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2795325
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