A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation



Titolo: Assessment of DNA damage by Micellar Electrokinetic Chromathography
Autori: 
FATTORINI, PAOLO
SORCABURU CIGLIERI, SOLANGE
mostra contributor esterni 
Data di pubblicazione: 2013
Serie: 
METHODS IN MOLECULAR BIOLOGY  
Abstract: A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation
Handle: http://hdl.handle.net/11368/2795325
Appare nelle tipologie:2.1 Contributo in Volume (Capitolo,Saggio)

File in questo prodotto:
Non ci sono file associati a questo prodotto.
Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/11368/2795325
  • Citazioni
  • ND
  • ND

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
 
 
 
©2014 University of Trieste dove siamo Privacy
Piazzale Europa,1 34127 Trieste, Italia - Tel. +39 040.558.7111 - P.IVA 00211830328 - C.F. 80013890324 - P.E.C.: ateneo@pec.units.it
 Copyright © 2020