A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation
Titolo: | Assessment of DNA damage by Micellar Electrokinetic Chromathography | |
Autori: | ||
Data di pubblicazione: | 2013 | |
Serie: | ||
Abstract: | A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of un-canonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR-templates used in several fields of investigation | |
Handle: | http://hdl.handle.net/11368/2795325 | |
Appare nelle tipologie: | 2.1 Contributo in Volume (Capitolo,Saggio) |
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