Currently, little is known about the cellular and molecular mechanisms that promote alveolar epithelial repair. A recent study suggests that Keratin14-expression could represent a marker of alveolar regeneration, since its expression was immunoistochemically detected in hyperplastic pneumocytes in human samples of ARDS-related DAD. Here we shown the analysis on lung samples of various human diseases in order to confirm the hyphotesis of the previous study. We used two molecular biology tecniques, Real Time Pcr and Western Blot, on autoptical and bioptical lung specimens. As a result, we demonstrate here that Krt14-expression is only found in pathological samples with alveolar regeneration or repair, while all normal control samples are Krt14 negative.
Keratin-14 expression as a marker of lung regeneration/repair. Conference Proceeding "CROSS-BORDER ITALY-SLOVENIA BIOMEDICAL RESEARCH: ARE WE READY FOR HORIZON 2020?"
Bussani Rossana;Confalonieri Marco
2014-01-01
Abstract
Currently, little is known about the cellular and molecular mechanisms that promote alveolar epithelial repair. A recent study suggests that Keratin14-expression could represent a marker of alveolar regeneration, since its expression was immunoistochemically detected in hyperplastic pneumocytes in human samples of ARDS-related DAD. Here we shown the analysis on lung samples of various human diseases in order to confirm the hyphotesis of the previous study. We used two molecular biology tecniques, Real Time Pcr and Western Blot, on autoptical and bioptical lung specimens. As a result, we demonstrate here that Krt14-expression is only found in pathological samples with alveolar regeneration or repair, while all normal control samples are Krt14 negative.Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
