Peptide microarrays are becoming a promising alternative to protein microarrays due to the challenges associated with protein immobilization and purification. Here, we put forward a novel experimental-based approach that combines DNA-directed immobilization, nanografting, and atomic force height measurements to immobilize computationally designed cyclic peptide on an ultra-flat gold substrate. This procedure yields peptide-DNA nanoarrays, which can bind to the solvent-exposed site on the Beta-2-microglobulin (β2m).
Spatially resolved peptide-DNA nanoassemblages for biomarker detection: A synergy of DNA-directed immobilization and nanografting / Adedeji, A.F., Ambrosetti, E., Casalis, L., Castronovo, M.. - 1811:(2018), pp. 151-162. [10.1007/978-1-4939-8582-1_10]
Spatially resolved peptide-DNA nanoassemblages for biomarker detection: A synergy of DNA-directed immobilization and nanografting
Ambrosetti E.;
2018-01-01
Abstract
Peptide microarrays are becoming a promising alternative to protein microarrays due to the challenges associated with protein immobilization and purification. Here, we put forward a novel experimental-based approach that combines DNA-directed immobilization, nanografting, and atomic force height measurements to immobilize computationally designed cyclic peptide on an ultra-flat gold substrate. This procedure yields peptide-DNA nanoarrays, which can bind to the solvent-exposed site on the Beta-2-microglobulin (β2m).Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
