the effects of water and hydrophobic solvents on the stability of the open and closed forms of R. miehei lipase were evaluated with different solvent models. Desolvation of arginine 86 at the water-lipid interface plays a key role in the activation process, while the contribution from hydrophobic stabilization of the open form is less important.
Modeling of Solvent Effects in the Activation of the Lipase from Rhizomucor Miehei
BENEDETTI, FABIO;BERTI, FEDERICO;LINDA, PAOLO;SABOT, ANDREA
1996-01-01
Abstract
the effects of water and hydrophobic solvents on the stability of the open and closed forms of R. miehei lipase were evaluated with different solvent models. Desolvation of arginine 86 at the water-lipid interface plays a key role in the activation process, while the contribution from hydrophobic stabilization of the open form is less important.File in questo prodotto:
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