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EnglishObjective: To characterize p53 and PCNA expression in primary tumors and lymph nodes metastases of oral/oropharynx carci- nomas by flow cytometry and to evaluate the relationship be- tween these parameters in primary tumors and locoregional metastases, clinical-hystopathological parameters and survival time. Methods: Twenty-eight patients were retrospectively studied: 13 with metastases and 15 without metastases. The parameters considered were the degree of keratinization, Broder’s grading of histological differentiation, invasive cell grading, thickness and tumor size. Nuclei were stained using anti-p53 and anti- PCNA FITC conjugated monoclonal antibodies. Was used a Coulter Epics Elite flow cytometer. Results: A close relationship between p53 and PCNA in pri- mary tumors; no correlation with clinical-histopathological parameters; no correlation between p53 and PCNA expression in primary tumors and in lymph nodes metastases; no corre- lation between p53 and PCNA values in primary tumors and survival. Conclusions: The different expression of p53 and PCNA in primary tumors and lymph nodes metastases is a questionable prognostic parameter.
| Titolo: | Correlation between p53 and PCNA in lymphnodes metastases of oral and oropharynx carcinomas |
| Autori: | |
| Data di pubblicazione: | 2007 |
| Stato di pubblicazione: | Pubblicato |
| Rivista: | |
| Abstract: | Objective: To characterize p53 and PCNA expression in primary tumors and lymph nodes metastases of oral/oropharynx carci- nomas by flow cytometry and to evaluate the relationship be- tween these parameters in primary tumors and locoregional metastases, clinical-hystopathological parameters and survival time. Methods: Twenty-eight patients were retrospectively studied: 13 with metastases and 15 without metastases. The parameters considered were the degree of keratinization, Broder’s grading of histological differentiation, invasive cell grading, thickness and tumor size. Nuclei were stained using anti-p53 and anti- PCNA FITC conjugated monoclonal antibodies. Was used a Coulter Epics Elite flow cytometer. Results: A close relationship between p53 and PCNA in pri- mary tumors; no correlation with clinical-histopathological parameters; no correlation between p53 and PCNA expression in primary tumors and in lymph nodes metastases; no corre- lation between p53 and PCNA values in primary tumors and survival. Conclusions: The different expression of p53 and PCNA in primary tumors and lymph nodes metastases is a questionable prognostic parameter. |
| Handle: | http://hdl.handle.net/11368/1767108 |
| Appare nelle tipologie: | 1.1 Articolo in Rivista |