The thermodynamics of the electron transfer (ET) process for beef heart and yeast cytochromes c and the Lys72Ala/Lys73Ala/Lys79Ala mutant of the latter species subjected to progressive urea-induced unfolding was determined electrochemically. The results indicate the presence of at least three protein forms which were assigned to a low-temperature and a high-temperature His- Met intermediate species and a bis-histidinate form (although the presence of a His-Lys form cannot be excluded)
Redox Thermodynamics of Cytochromes C Subjected to Urea Induced Unfolding / Stefano, Monari; Antonio, Ranieri; GIULIA DI, Rocco; GERT VAN DER, Zwan; Silvia, Peressini; Tavagnacco, Claudio; Diego, Millo; Marco, Borsari. - In: JOURNAL OF APPLIED ELECTROCHEMISTRY. - ISSN 0021-891X. - STAMPA. - 39:(2009), pp. 2181-2190. [10.1007/s10800-009-9804-7]
Redox Thermodynamics of Cytochromes C Subjected to Urea Induced Unfolding
TAVAGNACCO, CLAUDIO;
2009-01-01
Abstract
The thermodynamics of the electron transfer (ET) process for beef heart and yeast cytochromes c and the Lys72Ala/Lys73Ala/Lys79Ala mutant of the latter species subjected to progressive urea-induced unfolding was determined electrochemically. The results indicate the presence of at least three protein forms which were assigned to a low-temperature and a high-temperature His- Met intermediate species and a bis-histidinate form (although the presence of a His-Lys form cannot be excluded)Pubblicazioni consigliate
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