To investigate the short term effects of Doxycycline on MMP-2 and MMP-9.Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells.In plasma, MMP-9 activity was reduced (-22\%, 95\% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28\%, 95\% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52\%, 95\% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively).Doxy decreases MMP-9 plasma levels by around 20\%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.

Short term effects of doxycycline on matrix metalloproteinases 2 and 9.

FIOTTI, NICOLA;ZACCHIGNA, SERENA;GIACCA, MAURO;GRASSI, GABRIELE;GIANSANTE, CARLO
2009

Abstract

To investigate the short term effects of Doxycycline on MMP-2 and MMP-9.Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells.In plasma, MMP-9 activity was reduced (-22\%, 95\% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28\%, 95\% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52\%, 95\% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively).Doxy decreases MMP-9 plasma levels by around 20\%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2552535
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 19
social impact