Rabbit alveolar macrophages exposed to the ionophore A23187 in the presence of extracellular Ca2+ take up about 12 nmoles of cells. This uptake induces a slight, but significant, extracellular release of granule enzymes, β-glucuronidase and lysozyme, but not of the cytoplasmic marker, lactate dehydrogenase. If either EGTA is added to the medium or Mg2+ replaces Ca2+ no stimulation of secretion is observed. If the energy supply is decreased by treating the macrophages with mitochondrial inhibitors such as oligomycin, cyanide, or rotenone and antimycin, Ca2+-dependent secretion is potentiated several fold. Selective release of granule enzymes from macrophages exposed to A23187 and Ca2+ is also stimulated by cytochalasin B (CB).

Secretion of granule enzymes from alveolar macrophages. Regulation by intracellular Ca2+-buffering capacity.

GENNARO, RENATO;
1978

Abstract

Rabbit alveolar macrophages exposed to the ionophore A23187 in the presence of extracellular Ca2+ take up about 12 nmoles of cells. This uptake induces a slight, but significant, extracellular release of granule enzymes, β-glucuronidase and lysozyme, but not of the cytoplasmic marker, lactate dehydrogenase. If either EGTA is added to the medium or Mg2+ replaces Ca2+ no stimulation of secretion is observed. If the energy supply is decreased by treating the macrophages with mitochondrial inhibitors such as oligomycin, cyanide, or rotenone and antimycin, Ca2+-dependent secretion is potentiated several fold. Selective release of granule enzymes from macrophages exposed to A23187 and Ca2+ is also stimulated by cytochalasin B (CB).
http://www.sciencedirect.com/science/article/pii/0014482778902070
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2563250
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