The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by proteinmicroarray. The specificity for 6 of these antigens was confirmed by ELISA
Profiling celiac disease antibody repertoire / Sara, D'Angelo; Flavio, Mignone; Cecilia, Deantonio; Roberto Di, Niro; Roberta, Bordoni; Marzari, Roberto; Gianluca De, Bellis; Not, Tarcisio; Fortunato, Ferrara; Andrew, Bradbury; Claudio, Santoro; Sblattero, Daniele. - In: CLINICAL IMMUNOLOGY. - ISSN 1521-6616. - STAMPA. - 148:(2013), pp. 99-109. [10.1016/j.clim.2013.04.009]
Profiling celiac disease antibody repertoire
MARZARI, ROBERTO;NOT, TARCISIO;SBLATTERO, DANIELE
2013-01-01
Abstract
The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by proteinmicroarray. The specificity for 6 of these antigens was confirmed by ELISAPubblicazioni consigliate
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