The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly higher pH value compared to the Stevia extracts (F = 99.45, p < 0.01). Stevia extracts can be considered nonacidogenic.
Titolo: | An in vitro and in vivo Comparison of the Effect ofStevia rebaudianaExtracts on Different Caries-Related Variables: A Randomized Controlled Trial Pilot Study |
Autori: | |
Data di pubblicazione: | 2014 |
Rivista: | |
Abstract: | The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p < 0.01). After 5, 10, 15 and 30 min, the sucrose in vivo rinse produced a statistically significantly higher pH value compared to the Stevia extracts (F = 99.45, p < 0.01). Stevia extracts can be considered nonacidogenic. |
Handle: | http://hdl.handle.net/11368/2761568 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1159/000351650 |
Appare nelle tipologie: | 1.1 Articolo in Rivista |