Computed x-ray phase contrast micro-tomography is the most valuable tool for a three dimensional (3D) and non destructive analysis of the tissue engineered bone morphology. We used a Talbot interferometer installed at SYRMEP beamline of the ELETTRA synchrotron (Trieste, Italy) for a precise 3D reconstruction of both bone and soft connective tissue, regenerated in vivo within a porous scaffold. For the first time the x-ray tomographic reconstructions have been combined with x-ray scanning micro-diffraction measurement on the same sample, in order to give an exhaustive identification of the different tissues participating to the biomineralization process. As a result, we were able to investigate in detail the different densities in the tissues, distinguishing the 3D organization of the amorphous calcium phosphate from the collagen matrix. Our experimental approach allows for a deeper understanding of the role of collagen matrix in the organic-mineral transition, which is a crucial issue for the development of new bio-inspired composites.
Three dimensional visualization of engineered bone and soft tissue by combined x-ray micro-diffraction and phase contrast tomography / Alessia, C., Gaetano, C., Daniele, P., Inna, B., Michela, F., Manfred, B., Rigon, L., Arfelli, F., Rong Chang, C., Diego, D., Nicola, S., Sara, M., Giuliana, T., Ranieri, C., Maddalena, M.. - In: PHYSICS IN MEDICINE AND BIOLOGY. - ISSN 0031-9155. - 59:(2014), pp. 189-201. [10.1088/0031-9155/59/1/189]
Three dimensional visualization of engineered bone and soft tissue by combined x-ray micro-diffraction and phase contrast tomography
RIGON, LUIGI;ARFELLI, FULVIA;
2014-01-01
Abstract
Computed x-ray phase contrast micro-tomography is the most valuable tool for a three dimensional (3D) and non destructive analysis of the tissue engineered bone morphology. We used a Talbot interferometer installed at SYRMEP beamline of the ELETTRA synchrotron (Trieste, Italy) for a precise 3D reconstruction of both bone and soft connective tissue, regenerated in vivo within a porous scaffold. For the first time the x-ray tomographic reconstructions have been combined with x-ray scanning micro-diffraction measurement on the same sample, in order to give an exhaustive identification of the different tissues participating to the biomineralization process. As a result, we were able to investigate in detail the different densities in the tissues, distinguishing the 3D organization of the amorphous calcium phosphate from the collagen matrix. Our experimental approach allows for a deeper understanding of the role of collagen matrix in the organic-mineral transition, which is a crucial issue for the development of new bio-inspired composites.Pubblicazioni consigliate
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