The effect of different bile acids (BA) on the hepatic uptake of sulfobromophthalein (BSP) was investigated in liver plasma membrane vesicles enriched in basolateral fraction. BSP uptake was measured either in the absence (electroneutral component) or in the presence of a membrane potential (electrogenic component) induced by the addition of valinomycin in the presence of an inwardly-directed potassium gradient. BSP uptake was also measured in the presence of different BA [cholate (C), taurocholate (TC), ursodeoxycholate (UDC) and tauroursodeoxycholate (TUDC)]. Electrogenic BSP uptake was not affected by BA. Conversely, the electroneutral portion of the BSP uptake was inhibited with an inhibition constant (Ki, microM) of 230 +/- 40 for C, 103 +/- 33 for TC, 99 +/- 34 for UDC and 120 +/- 39 for TUDC, respectively (means +/- SD, N = 4). The Dixon and Cornish-Bowden plot of the data revealed an uncompetitive type of inhibition for each BA. These data indicate that the electroneutral, but not the electrogenic, BSP transport system is modulated by BA.
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