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EnglishHepatocytic transport of physiological concentrations of unconjugated bilirubin (UCB) has not been determined in isolated liver cells. Initial uptake of highly purified [3H]UCB was measured in rat hepatocytes in the presence of human serum albumin at various free, unbound UCB concentrations, [UCB]. At [UCB]=42 nM (below aqueous solubility of 70 nM), uptake was strictly temperature dependent; this was much less evident at [UCB]=166 nM (supersaturated). At low, physiological UCB concentrations, specific UCB uptake showed saturative kinetics with an apparent Km of 41 nM, indicating carrier-mediated transport. With aqueous supersaturation, UCB entered hepatocytes mainly by passive diffusion.
| Titolo: | Uptake of [3H]bilirubin in freshly isolated rat hepatocytes: role of free bilirubin concentration |
| Autori: | |
| Data di pubblicazione: | 1999 |
| Rivista: | |
| Abstract: | Hepatocytic transport of physiological concentrations of unconjugated bilirubin (UCB) has not been determined in isolated liver cells. Initial uptake of highly purified [3H]UCB was measured in rat hepatocytes in the presence of human serum albumin at various free, unbound UCB concentrations, [UCB]. At [UCB]=42 nM (below aqueous solubility of 70 nM), uptake was strictly temperature dependent; this was much less evident at [UCB]=166 nM (supersaturated). At low, physiological UCB concentrations, specific UCB uptake showed saturative kinetics with an apparent Km of 41 nM, indicating carrier-mediated transport. With aqueous supersaturation, UCB entered hepatocytes mainly by passive diffusion. |
| Handle: | http://hdl.handle.net/11368/2852940 |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0014-5793(99)01606-3 |
| URL: | http://www.sciencedirect.com/science/article/pii/S0014579399016063 |
| Appare nelle tipologie: | 1.1 Articolo in Rivista |