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EnglishWhile reactive oxygen species (ROS) play a role in muscle repair, excessive amounts of ROS for extended periods may lead to oxidative stress. Antioxidants, as resveratrol (RS), may reduce oxidative stress, restore mitochondrial function and promote myogenesis and hypertrophy. However, RS dose-effectiveness for muscle plasticity is unclear. Therefore, we investigated RS dose-response on C2C12 myoblast and myotube plasticity 1. in the presence and 2. absence of different degrees of oxidative stress. Low RS concentration (10 μM) stimulated myoblast cell cycle arrest, migration and sprouting, which were inhibited by higher doses (40-60 μM). RS did not increase oxidative capacity. In contrast, RS induced mitochondria loss, reduced cell viability and ROS production, and activated stress response pathways [Hsp70 and pSer36-p66(ShcA) proteins]. However, the deleterious effects of H2O2 (1000 µM) on cell migration were alleviated after preconditioning with 10 µM-RS. This dose also enhanced cell motility mediated by 100 µM-H2O2, while higher RS-doses augmented the H2O2-induced impaired myoblast regeneration and mitochondrial dehydrogenase activity. In conclusion, low resveratrol doses promoted in vitro muscle regeneration and attenuated the impact of ROS, while high doses augmented the reduced plasticity and metabolism induced by oxidative stress. Thus, the effects of resveratrol depend on its dose and degree of oxidative stress.
| Titolo: | The impact of resveratrol and hydrogen peroxide on muscle cell plasticity shows a dose-dependent interaction. |
| Autori: | |
| Data di pubblicazione: | 2015 |
| Rivista: | |
| Abstract: | While reactive oxygen species (ROS) play a role in muscle repair, excessive amounts of ROS for extended periods may lead to oxidative stress. Antioxidants, as resveratrol (RS), may reduce oxidative stress, restore mitochondrial function and promote myogenesis and hypertrophy. However, RS dose-effectiveness for muscle plasticity is unclear. Therefore, we investigated RS dose-response on C2C12 myoblast and myotube plasticity 1. in the presence and 2. absence of different degrees of oxidative stress. Low RS concentration (10 μM) stimulated myoblast cell cycle arrest, migration and sprouting, which were inhibited by higher doses (40-60 μM). RS did not increase oxidative capacity. In contrast, RS induced mitochondria loss, reduced cell viability and ROS production, and activated stress response pathways [Hsp70 and pSer36-p66(ShcA) proteins]. However, the deleterious effects of H2O2 (1000 µM) on cell migration were alleviated after preconditioning with 10 µM-RS. This dose also enhanced cell motility mediated by 100 µM-H2O2, while higher RS-doses augmented the H2O2-induced impaired myoblast regeneration and mitochondrial dehydrogenase activity. In conclusion, low resveratrol doses promoted in vitro muscle regeneration and attenuated the impact of ROS, while high doses augmented the reduced plasticity and metabolism induced by oxidative stress. Thus, the effects of resveratrol depend on its dose and degree of oxidative stress. |
| Handle: | http://www.nature.com/srep/2015/150128/srep08093/full/srep08093.html http://hdl.handle.net/11368/2863917 |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1038/srep08093 |
| URL: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308712/pdf/srep08093.pdf |
| Appare nelle tipologie: | 1.1 Articolo in Rivista |
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| The impact of resveratrol and hydrogen peroxide on muscle cell plasticity shows a dose-dependent interaction.pdf | pdf articolo | Documento in Versione Editoriale | Digital Rights Management non definito | Open Access Visualizza/Apri |
| The impact of resveratrol and hydrogen...-Supplementary information.pdf | pdf materiale aggiuntivo | Documento in Versione Editoriale | Digital Rights Management non definito | Open Access Visualizza/Apri |
| The impact of resveratrol and hydrogen...-Corrigendum.pdf | pdf errata corrige | Documento in Versione Editoriale | Digital Rights Management non definito | Open Access Visualizza/Apri |
