Her2 protein is the key marker determining the choice of Herceptin therapy after a diagnosis of breast cancer. Its evaluation is made in most laboratories by immunohistochemistry, and interpreted by a pathologist using an optical microscope, a process subject to inter-observer variability, particularly for samples scored as equivocal (2+). Software analysis products have been introduced, seeking to reduce this variability. In this study, we compared the results of both traditional evaluation and a specific software package (VISIA Imaging) to those from fluorescent in situ hybridization (FISH).

Her2 immunohistochemical evaluation by traditional microscopy and by digital analysis, and the consequences for FISH testing

MARCUZZO, THOMAS;GIUDICI, FABIOLA;RIZZARDI, CLARA;BOTTIN, CRISTINA;ZANCONATI, FABRIZIO
2016

Abstract

Her2 protein is the key marker determining the choice of Herceptin therapy after a diagnosis of breast cancer. Its evaluation is made in most laboratories by immunohistochemistry, and interpreted by a pathologist using an optical microscope, a process subject to inter-observer variability, particularly for samples scored as equivocal (2+). Software analysis products have been introduced, seeking to reduce this variability. In this study, we compared the results of both traditional evaluation and a specific software package (VISIA Imaging) to those from fluorescent in situ hybridization (FISH).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2882446
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