A rapid and reliable protocol was developed to produce chemical degradation of the RNA in vitro. Cell line MDA-MB-453 was used as source of RNA and hydrolysis in water at 70 C was performed. The outcome was monitored by RT-qPCR of two housekeeping targets and a significant correlation between the Ct and the extent of molecular damage of the template was found. A molecular degradation index of the RNA is proposed.

Reliability of RT-qPCR from degraded RNA samples: An in vitro model

BONIN, Serena;NICOLIN, VANESSA;FATTORINI, PAOLO
2015

Abstract

A rapid and reliable protocol was developed to produce chemical degradation of the RNA in vitro. Cell line MDA-MB-453 was used as source of RNA and hydrolysis in water at 70 C was performed. The outcome was monitored by RT-qPCR of two housekeeping targets and a significant correlation between the Ct and the extent of molecular damage of the template was found. A molecular degradation index of the RNA is proposed.
FORENSIC SCIENCE INTERNATIONAL: GENETICS SUPPLEMENT SERIES
http://www.fsigeneticssup.com/article/S1875-1768(15)30017-2/pdf
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2885144
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