We investigate the impact of an over-looked component on molecular recognition in water–buffer. The binding of a cationic dye to biological polyanion heparin is shown by isothermal calorimetry to depend on buffer (Tris–HCl 4 HEPES 4 PBS). The heparin binding of selfassembled multivalent (SAMul) cationic micelles is even more buffer dependent. Multivalent electrostatic molecular recognition is buffer dependent as a result of competitive interactions between the cationic binding interface and anions present in the buffer.

Effect of buffer at nanoscale molecular recognition interfaces – electrostatic binding of biological polyanions

LAURINI, ERIK;PRICL, SABRINA;
2017-01-01

Abstract

We investigate the impact of an over-looked component on molecular recognition in water–buffer. The binding of a cationic dye to biological polyanion heparin is shown by isothermal calorimetry to depend on buffer (Tris–HCl 4 HEPES 4 PBS). The heparin binding of selfassembled multivalent (SAMul) cationic micelles is even more buffer dependent. Multivalent electrostatic molecular recognition is buffer dependent as a result of competitive interactions between the cationic binding interface and anions present in the buffer.
2017
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http://pubs.rsc.org/en/Content/ArticleLanding/2017/CC/C7CC07413A#!divAbstract
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11368/2911142
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