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EnglishProstate-specific membrane antigen (PSMA), a glycoprotein expressed in the prostatic epithelium endowed with enzymatic activity, is a very promising diagnostic marker for the early detection of prostate cancer. In this study, we report a novel electrochemiluminescence ELISA-like immunosensor based on carbon nanotubes and a highly specific sandwich immunoassay for the PSMA detection. To fabricate the device, an optically transparent electrode was modified with doubly functionalized multi-walled carbon nanotubes carrying amine groups and a monoclonal anti-PSMA antibody. Subsequently, to complete the sandwich immunosensing device, a second specific monoclonal anti-PSMA antibody was labelled with a electrochemiluminescent probe. Under optimized experimental conditions, the proposed sensing device exhibits a performance exceeding that of the state of-the-art in terms of the limit of detection (LOD) and limit of quantification (LOQ) as good as 0.88 ng mL−1 and 2.60 ng mL−1, respectively, in real complex samples such as cell lysates. In addition, the unique role of carbon nanotubes is also discussed by comparison with an analogue sensor assembled without the nanocarbon-based material.
| Titolo: | Highly sensitive electrochemiluminescence detection of a prostate cancer biomarker | |
| Autori: | PRATO, MAURIZIO (Corresponding) | |
| Data di pubblicazione: | 2017 | |
| Stato di pubblicazione: | Pubblicato | |
| Rivista: | ||
| Abstract: | Prostate-specific membrane antigen (PSMA), a glycoprotein expressed in the prostatic epithelium endowed with enzymatic activity, is a very promising diagnostic marker for the early detection of prostate cancer. In this study, we report a novel electrochemiluminescence ELISA-like immunosensor based on carbon nanotubes and a highly specific sandwich immunoassay for the PSMA detection. To fabricate the device, an optically transparent electrode was modified with doubly functionalized multi-walled carbon nanotubes carrying amine groups and a monoclonal anti-PSMA antibody. Subsequently, to complete the sandwich immunosensing device, a second specific monoclonal anti-PSMA antibody was labelled with a electrochemiluminescent probe. Under optimized experimental conditions, the proposed sensing device exhibits a performance exceeding that of the state of-the-art in terms of the limit of detection (LOD) and limit of quantification (LOQ) as good as 0.88 ng mL−1 and 2.60 ng mL−1, respectively, in real complex samples such as cell lysates. In addition, the unique role of carbon nanotubes is also discussed by comparison with an analogue sensor assembled without the nanocarbon-based material. | |
| Handle: | http://hdl.handle.net/11368/2919660 | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1039/c7tb01557g | |
| URL: | http://pubs.rsc.org/en/Content/ArticleLanding/2017/TB/C7TB01557G | |
| Appare nelle tipologie: | 1.1 Articolo in Rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| J Mat Chem B 2017.pdf | Documento in Versione Editoriale | Copyright Editore | Administrator Richiedi una copia | |
| c7tb01557g1.pdf | Supplementary information | Altro materiale allegato | Copyright Editore | Administrator Richiedi una copia |
http://hdl.handle.net/11368/2919660
