OP28 The complement component C3 is expressed by the endometrial ectopic tissue and is involved in the endometriotic lesion formation C. Agostinis 1, G. Zito 1, D. De Santo1, R. Vidergar2, O. Radillo 1, F. Bossi 1, S. Zorzet2, G. Ricci 1, R. Bulla 2 1 Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Trieste, Italy 2 Department of Life Sciences, University of Trieste, Trieste, Italy E-mail address: chiara.agostinis@burlo.trieste.it (C. Agostinis). Background: The complement (C) system is one of the major components of humoral innate immunity, acting as the first lines of defence against microbes. The principal roles of C system are the opsonization and lysis of pathogens, but new roles in inflammatory and immunological processes are emerging. It is involved in numerous inflammatory diseases, such as SLE, PNH and endometriosis (EM). Several groups have been demonstrated that the glandular epithelial cells found in endometriotic implants produce and secrete the C component C3. The aim of this work was to confirm the presence of C3 the in the ectopic tissue compared to the eutopic one, and investigate the role of C3 in the pathogenesis of EM. Methods:Weinvestigated by immunofluorescence, the expression of C3 on sections of endometriotic cysts and healthy uterus; we performed RT-qPCR experiments to highlight the synthesis of this C component at local level. We set up a murine in vivo model of endometriosis based on the injection of minced uterine tissue from a donor mouse, into the peritoneum of a receiving animal. Results: We confirmed the presence of C3 selectively in the ectopic and not in eutopic endometrium, and the local synthesis of C3 in endometriotic tissue. We observed a greater amount of cyst formation in the peritoneum of WT mice compared to C3 KO mice.Conclusion: We concluded that C3 can actually be considered a marker of EM and that the local synthesis of this C component can promote the engraftment of the cysts.

The complement component C3 is expressed by the endometrial ectopic tissue and is involved in the endometriotic lesion formation

Agostinis, C.;Zito, G.;De Santo, D.;Vidergar, R.;Radillo, O.;Bossi, F.;Zorzet, S.;Ricci, G.;Bulla, R.
2018

Abstract

OP28 The complement component C3 is expressed by the endometrial ectopic tissue and is involved in the endometriotic lesion formation C. Agostinis 1, G. Zito 1, D. De Santo1, R. Vidergar2, O. Radillo 1, F. Bossi 1, S. Zorzet2, G. Ricci 1, R. Bulla 2 1 Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Trieste, Italy 2 Department of Life Sciences, University of Trieste, Trieste, Italy E-mail address: chiara.agostinis@burlo.trieste.it (C. Agostinis). Background: The complement (C) system is one of the major components of humoral innate immunity, acting as the first lines of defence against microbes. The principal roles of C system are the opsonization and lysis of pathogens, but new roles in inflammatory and immunological processes are emerging. It is involved in numerous inflammatory diseases, such as SLE, PNH and endometriosis (EM). Several groups have been demonstrated that the glandular epithelial cells found in endometriotic implants produce and secrete the C component C3. The aim of this work was to confirm the presence of C3 the in the ectopic tissue compared to the eutopic one, and investigate the role of C3 in the pathogenesis of EM. Methods:Weinvestigated by immunofluorescence, the expression of C3 on sections of endometriotic cysts and healthy uterus; we performed RT-qPCR experiments to highlight the synthesis of this C component at local level. We set up a murine in vivo model of endometriosis based on the injection of minced uterine tissue from a donor mouse, into the peritoneum of a receiving animal. Results: We confirmed the presence of C3 selectively in the ectopic and not in eutopic endometrium, and the local synthesis of C3 in endometriotic tissue. We observed a greater amount of cyst formation in the peritoneum of WT mice compared to C3 KO mice.Conclusion: We concluded that C3 can actually be considered a marker of EM and that the local synthesis of this C component can promote the engraftment of the cysts.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2928858
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