This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0-36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0-10 h in a previous study.
Prolonged DNA hydrolysis in water: A study on DNA stability / Fattorini, P., Marrubini, G., Bonin, S., Bertoglio, B., Grignani, P., Recchia, E., Pitacco, P., Procopio, F., Cantoni, C., Pajnič, I.z., Sorçaburu-Cigliero, S., Previdere, C.. - In: DATA IN BRIEF. - ISSN 2352-3409. - ELETTRONICO. - 20:(2018), pp. 1237-1243. [10.1016/j.dib.2018.08.120]
Prolonged DNA hydrolysis in water: A study on DNA stability
Fattorini P
;Bonin S;Pitacco P;Procopio F;Cantoni C;
2018-01-01
Abstract
This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0-36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0-10 h in a previous study.| File | Dimensione | Formato | |
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