The investigation of cellular mechanical properties for the evaluation of the cell physiological state has emerged as a hot topic in the last decade. In this framework, different aspects of the mechanobiology are considered in three biomedical fields. First, the alteration of the mechanical phenotype, the cell structure and morphology of melanoma cells according to the levels of production of a factor involved in the cytoskeleton organization, are considered. Secondly, mechanotransduction, and more precisely the capability of cells to adapt their mechanics to the environmental condition was investigated on the effect of a heart failure on cardiac pericytes. In the last part, the mechanical properties of oocytes have been identified as a scoring system to evaluate the quality of oocytes to be selected for the practice of the in vitro fertilization. In particular, I investigated the evolution of the oocyte stiffness and viscosity during post-ovulatory ageing, one of the processes responsible for the decreased yields of in vitro fertilization. Here, two mechanical parameters were found, able to predict ageing status of the oocytes before any visual feature due to degradation. allowing to introduce a novel classification for pre-apoptotic and non-fertile oocytes.

The investigation of cellular mechanical properties for the evaluation of the cell physiological state has emerged as a hot topic in the last decade. In this framework, different aspects of the mechanobiology are considered in three biomedical fields. First, the alteration of the mechanical phenotype, the cell structure and morphology of melanoma cells according to the levels of production of a factor involved in the cytoskeleton organization, are considered. Secondly, mechanotransduction, and more precisely the capability of cells to adapt their mechanics to the environmental condition was investigated on the effect of a heart failure on cardiac pericytes. In the last part, the mechanical properties of oocytes have been identified as a scoring system to evaluate the quality of oocytes to be selected for the practice of the in vitro fertilization. In particular, I investigated the evolution of the oocyte stiffness and viscosity during post-ovulatory ageing, one of the processes responsible for the decreased yields of in vitro fertilization. Here, two mechanical parameters were found, able to predict ageing status of the oocytes before any visual feature due to degradation. allowing to introduce a novel classification for pre-apoptotic and non-fertile oocytes.

Atomic Force Microscopy-based essay for biomedical applications / Battistella, Alice. - (2021 Sep 17).

Atomic Force Microscopy-based essay for biomedical applications

BATTISTELLA, ALICE
2021-09-17

Abstract

The investigation of cellular mechanical properties for the evaluation of the cell physiological state has emerged as a hot topic in the last decade. In this framework, different aspects of the mechanobiology are considered in three biomedical fields. First, the alteration of the mechanical phenotype, the cell structure and morphology of melanoma cells according to the levels of production of a factor involved in the cytoskeleton organization, are considered. Secondly, mechanotransduction, and more precisely the capability of cells to adapt their mechanics to the environmental condition was investigated on the effect of a heart failure on cardiac pericytes. In the last part, the mechanical properties of oocytes have been identified as a scoring system to evaluate the quality of oocytes to be selected for the practice of the in vitro fertilization. In particular, I investigated the evolution of the oocyte stiffness and viscosity during post-ovulatory ageing, one of the processes responsible for the decreased yields of in vitro fertilization. Here, two mechanical parameters were found, able to predict ageing status of the oocytes before any visual feature due to degradation. allowing to introduce a novel classification for pre-apoptotic and non-fertile oocytes.
17-set-2021
LAZZARINO, MARCO
33
2019/2020
Settore FIS/03 - Fisica della Materia
Università degli Studi di Trieste
File in questo prodotto:
File Dimensione Formato  
Thesis Final_afterRevision.pdf

accesso aperto

Descrizione: ATOMIC FORCE MICROSCOPY-BASED ESSAY FOR BIOMEDICAL APPLICATIONS
Tipologia: Tesi di dottorato
Dimensione 5.56 MB
Formato Adobe PDF
5.56 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11368/2996075
 Avviso

Registrazione in corso di verifica.
La registrazione di questo prodotto non è ancora stata validata in ArTS.

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact