The neurotrophin brain-derived neurotrophic factor (BDNF) plays a key role in neuronal survival and neurite out-growth, synaptogenesis and synaptic plasticity. BDNF mRNA can be transported in neuronal dendrites in an activity-dependent manner in particular, following seizures but also in response to antidepressants or physical activity. At present, a clear demonstration that BDNF mRNA is locally transported and translated at activated synapses in response to the induction of long-term potentiation (LTP) is still lacking. Here, we study the dynamics of BDNF mRNA trafficking during neuronal plasticity induced by chemical-LTP. The project explores the two hypotheses of selective vs. non-selective dendritic transport of BDNF transcripts after synaptic potentiation and the related methodological constrains using the MS2 system for mRNA visualization in living neurons

The neurotrophin brain-derived neurotrophic factor (BDNF) plays a key role in neuronal survival and neurite out-growth, synaptogenesis and synaptic plasticity. BDNF mRNA can be transported in neuronal dendrites in an activity-dependent manner in particular, following seizures but also in response to antidepressants or physical activity. At present, a clear demonstration that BDNF mRNA is locally transported and translated at activated synapses in response to the induction of long-term potentiation (LTP) is still lacking. Here, we study the dynamics of BDNF mRNA trafficking during neuronal plasticity induced by chemical-LTP. The project explores the two hypotheses of selective vs. non-selective dendritic transport of BDNF transcripts after synaptic potentiation and the related methodological constrains using the MS2 system for mRNA visualization in living neurons.

Local transport and translation of BDNF following synaptic potentiation

BIMBI, GIORGIA
2023-03-24

Abstract

The neurotrophin brain-derived neurotrophic factor (BDNF) plays a key role in neuronal survival and neurite out-growth, synaptogenesis and synaptic plasticity. BDNF mRNA can be transported in neuronal dendrites in an activity-dependent manner in particular, following seizures but also in response to antidepressants or physical activity. At present, a clear demonstration that BDNF mRNA is locally transported and translated at activated synapses in response to the induction of long-term potentiation (LTP) is still lacking. Here, we study the dynamics of BDNF mRNA trafficking during neuronal plasticity induced by chemical-LTP. The project explores the two hypotheses of selective vs. non-selective dendritic transport of BDNF transcripts after synaptic potentiation and the related methodological constrains using the MS2 system for mRNA visualization in living neurons.
24-mar-2023
TONGIORGI, Enrico
35
2021/2022
Settore BIO/06 - Anatomia Comparata e Citologia
Università degli Studi di Trieste
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11368/3042423
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