The distance between Ca(V)2.1 voltage-gated Ca2+ channels and the Ca2+ sensor responsible for vesicle release at presynaptic terminals is critical for determining synaptic strength. Yet, the molecular mechanisms responsible for a loose coupling configuration of CaV2.1 in certain synapses or developmental periods and a tight one in others remain unknown. Here, we examine the nanoscale organization of two Ca(V)2.1 splice isoforms (Ca(V)2.1[EFa] and Ca(V)2.1[EFb]) at presynaptic terminals by superresolution structured illumination microscopy. We find that Ca(V)2.1[EFa] is more tightly co-localized with presynaptic markers than Ca(V)2.1[EFb], suggesting that alternative splicing plays a crucial role in the synaptic organization of Ca(V)2.1 channels.

Nanoscale organization of CaV2.1 splice isoforms at presynaptic terminals: implications for synaptic vesicle release and synaptic facilitation

Cingolani, Lorenzo A.
;
Thalhammer, Agnes
;
Jaudon, Fanny;Muià, Jessica;Baj, Gabriele
2023-01-01

Abstract

The distance between Ca(V)2.1 voltage-gated Ca2+ channels and the Ca2+ sensor responsible for vesicle release at presynaptic terminals is critical for determining synaptic strength. Yet, the molecular mechanisms responsible for a loose coupling configuration of CaV2.1 in certain synapses or developmental periods and a tight one in others remain unknown. Here, we examine the nanoscale organization of two Ca(V)2.1 splice isoforms (Ca(V)2.1[EFa] and Ca(V)2.1[EFb]) at presynaptic terminals by superresolution structured illumination microscopy. We find that Ca(V)2.1[EFa] is more tightly co-localized with presynaptic markers than Ca(V)2.1[EFb], suggesting that alternative splicing plays a crucial role in the synaptic organization of Ca(V)2.1 channels.
2023
4-set-2023
Pubblicato
https://www.degruyter.com/document/doi/10.1515/hsz-2023-0235/html
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11368/3070020
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