A single DNA sample underwent controlled damaging treatments represented by UV radiations, free radicals, formaldehyde, reactive oxygen species (ROS), metal ions radical starters such as Fe3+ and Cu2+, HCl, and NaOH. Capillary electrophoresis showed that most of the treatments produced chemical alteration of the primary structure of the DNAs. In addition, both Alu probing and real-time PCR provided data lower than the controls. Both STR typing and Y-specific SNPs analysis were influenced by the altered composition of the templates. The results of this study indicate that more information on the molecular composition of the template can be extremely useful for the evaluation of the PCR-based evidences. © 2006.
Molecular analysis of in vitro damaged DNA samples / Fattorini, P., Tomasella, F., Grignani, P., Sanchez-Diz, P., Ricci, U., Carracedo, A., Previdere, C.. - In: INTERNATIONAL CONGRESS SERIES. - ISSN 0531-5131. - 1288:(2006), pp. 704-706. [10.1016/j.ics.2005.12.009]
Molecular analysis of in vitro damaged DNA samples
Fattorini P.
;
2006-01-01
Abstract
A single DNA sample underwent controlled damaging treatments represented by UV radiations, free radicals, formaldehyde, reactive oxygen species (ROS), metal ions radical starters such as Fe3+ and Cu2+, HCl, and NaOH. Capillary electrophoresis showed that most of the treatments produced chemical alteration of the primary structure of the DNAs. In addition, both Alu probing and real-time PCR provided data lower than the controls. Both STR typing and Y-specific SNPs analysis were influenced by the altered composition of the templates. The results of this study indicate that more information on the molecular composition of the template can be extremely useful for the evaluation of the PCR-based evidences. © 2006.Pubblicazioni consigliate
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