Surface-Enhanced Raman Scattering of Bioaerosol: Where Are We Now? A Systematic Review

Surface-enhanced Raman scattering (SERS) spectroscopy has grown in popularity as a bioaerosol monitoring method due to its high sensitivity and specificity, as well as its ability to be performed in complex biological mixtures using portable and relatively inexpensive devices. However, due to a lack of standardised methodologies, SERS sensing of bioaerosols remains difficult. Full-length peer-reviewed journal articles related to the application of SERS spectroscopy to examine bioaerosols were systematically searched in PubMed, Scopus, and Web of Science databases using the PRISMA guidelines. A total of 13 studies met the inclusion criteria for our systematic literature search. A critical evaluation of the experimental aspects involved in the collection of bioaerosols for SERS analysis is presented, as well as the elective applicability and weaknesses of various experimental setups, helping to provide a solid foundation for real-time bioaerosol characterisation using SERS spectroscopy.