Despite growinginterestintheroleofthereproductivemicrobiota,theuterinemicrobiotaofmareshasbeenonlyscarcely investigated usingsequencingapproaches.Theaimsofthisstudyweretodescribetheuterinemicrobiotaofmareswithen- dometritis andthee7ectsofantibiotictreatmentusingboth16SrRNAsequencingandculture.Fivemareswithclinicalsignsof endometritis andapositivebacteriologicalculturewereenrolled.Duringthefollicularphase(T0),uterinesampleswerecollected using adouble-guardedcytobrushandswabformicrobiomeandbacteriologicalanalysis,respectively.Followingtheantimicrobial susceptibility test,theyweretreatedwithintrauterineinfusionsofceftiofursodium,andsamplingswererepeatedduringthe subsequent follicularphase(T1).Accordingtobacterialculture,atT0, Streptococcus equizooepidemicus was identi8edin4mares and Escherichia coli in one.AtT1,3maresresultednegative,onewaspositivefor Staphylococcus xylosus, andonecontinuedtotest positive for E. coli. According toNGS,themostrepresentedgeneraatT0were Streptococcus, Escherichia-Shigella, Corynebacterium, Arcanobacterium, Porphyromonas, andStaphylococcus. The8rst3generadominatedthemicrobiomeof4mareswitharelative abundance rangingfrom44%to99%.AtT1,thesegenera’srelativeabundancedropped,andthemostabundantwere Acinetobacter, Staphylococcus, and Pseudomonas. Furthermore, afterintrauterineinfusionofceftiofur,themicrobiomewasmorediverse, according toShannonandSimpsonindexes(p < 0.05).
The Uterine Microbiota in Mares With Endometritis: Impacts of Antibiotic Treatment / Donato, Gian Guido; Nebbia, Patrizia; Stella, Maria Cristina; Gionechetti, Fabrizia; Ala, Ugo; Cristofoli, Davide; Robino, Patrizia; Pallavicini, Alberto; Nervo, Tiziana. - In: VETERINARY MEDICINE INTERNATIONAL (PRINT). - ISSN 2090-8113. - ELETTRONICO. - 2026:1(2026), pp. ---. [10.1155/vmi/5270993]
The Uterine Microbiota in Mares With Endometritis: Impacts of Antibiotic Treatment
Gionechetti, Fabrizia;Pallavicini, Alberto;
2026-01-01
Abstract
Despite growinginterestintheroleofthereproductivemicrobiota,theuterinemicrobiotaofmareshasbeenonlyscarcely investigated usingsequencingapproaches.Theaimsofthisstudyweretodescribetheuterinemicrobiotaofmareswithen- dometritis andthee7ectsofantibiotictreatmentusingboth16SrRNAsequencingandculture.Fivemareswithclinicalsignsof endometritis andapositivebacteriologicalculturewereenrolled.Duringthefollicularphase(T0),uterinesampleswerecollected using adouble-guardedcytobrushandswabformicrobiomeandbacteriologicalanalysis,respectively.Followingtheantimicrobial susceptibility test,theyweretreatedwithintrauterineinfusionsofceftiofursodium,andsamplingswererepeatedduringthe subsequent follicularphase(T1).Accordingtobacterialculture,atT0, Streptococcus equizooepidemicus was identi8edin4mares and Escherichia coli in one.AtT1,3maresresultednegative,onewaspositivefor Staphylococcus xylosus, andonecontinuedtotest positive for E. coli. According toNGS,themostrepresentedgeneraatT0were Streptococcus, Escherichia-Shigella, Corynebacterium, Arcanobacterium, Porphyromonas, andStaphylococcus. The8rst3generadominatedthemicrobiomeof4mareswitharelative abundance rangingfrom44%to99%.AtT1,thesegenera’srelativeabundancedropped,andthemostabundantwere Acinetobacter, Staphylococcus, and Pseudomonas. Furthermore, afterintrauterineinfusionofceftiofur,themicrobiomewasmorediverse, according toShannonandSimpsonindexes(p < 0.05).Pubblicazioni consigliate
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