Bacteria collection from static millilitre sample using a DC field

Efficient bacterial collection from millilitre-scale liquid samples remains challenging for preconcentrationbased analyses. We present a device that uses a DC electric field to transport and deposit bacteria onto an agarose pad in a static chamber. The system operates at physiological ionic strength while preserving cell viability by limiting electrochemical reactions. Accumulation of live bacteria at low concentrations (10³ CFU/mL) is demonstrated at 5V. Transport, viability, and collection efficiency are confirmed by microscopy and plate assays. This approach enables rapid bacterial pre-enrichment and detection.