Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna un-guiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.
Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.) / Padovan, Lara; Segat, Ludovica; Tossi, Alessandro; T., Calsa; A. K., Ederson; L., Brandao; R. L., Guimarães; V., Pandolfi; M. C., Pestana Calsa; L. C., Belarmino; A. M., Benko Iseppon; Crovella, Sergio. - In: PROTEIN AND PEPTIDE LETTERS. - ISSN 0929-8665. - STAMPA. - 17:3(2010), pp. 297-304. [10.2174/092986610790780350]
Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.).
PADOVAN, LARA;SEGAT, LUDOVICA;TOSSI, ALESSANDRO;CROVELLA, SERGIO
2010-01-01
Abstract
Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna un-guiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.Pubblicazioni consigliate
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