Background: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a con- dition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals. Aims: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD). Methods: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines – interleukin (IL)- 15, tumor necrosis factor-?, IL-1?, IL-6, IL-12p70, IL-23, IL-27, IL-32?, thymic stromal lymphopoietin (TSLP), IFN-?-, adaptive cytokines – interferon (IFN)-?, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines – IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Results: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-?, IFN-?, IL-17A, IL- 23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls. Conclusion: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.

Innate and adaptive immunity in self-reported nonceliac gluten sensitivity versus celiac disease

NAVIGLIO, SAMUELE;DE LEO, LUIGINA;NOT, TARCISIO;
2016

Abstract

Background: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a con- dition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals. Aims: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD). Methods: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines – interleukin (IL)- 15, tumor necrosis factor-?, IL-1?, IL-6, IL-12p70, IL-23, IL-27, IL-32?, thymic stromal lymphopoietin (TSLP), IFN-?-, adaptive cytokines – interferon (IFN)-?, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines – IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Results: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-?, IFN-?, IL-17A, IL- 23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls. Conclusion: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.
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http://www.sciencedirect.com/science/article/pii/S159086581530428X
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Descrizione: Post Print VQR3 - This is an Accepted Manuscript of an article published by Elsevier in Digestive and Liver Disease on 7 Apr 2016, available online: https://doi.org/10.1016/j.dld.2016.03.024
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11368/2880923
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