Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa

Baba, Marycelin Mandu; Bitew, Molalegne; Fokam, Joseph; Lelo, Eric Agola; Ahidjo, Ahmed; Asmamaw, Kominist; Beloumou, Grace Angong; Bulimo, Wallace Dimbuson; Buratti, Emanuele; Chenwi, Collins; Dadi, Hailu; D'Agaro, Pierlanfranco; De Conti, Laura; Fainguem, Nadine; Gadzama, Galadima; Maiuri, Paolo; Majanja, Janet; Meshack, Wadegu; Ndjolo, Alexis; Nkenfou, Celine; Oderinde, Bamidele Soji; Opanda, Silvanos Mukunzi; Segat, Ludovica; Stuani, Cristiana; Symekher, Samwel L; Takou, Desire; Tesfaye, Kassahun; Triolo, Gianluca; Tuki, Keyru; Zacchigna, Serena; Marcello, Alessandro

doi:10.1016/j.eclinm.2021.101101

Management and control of the COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus SARS-CoV-2 is critically dependent on quick and reliable identification of the virus in clinical specimens. Detection of viral RNA by a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a simple, reliable and cost-effective assay, deployable in resource-limited settings (RLS). Our objective was to evaluate the intrinsic and extrinsic performances of RT-LAMP in RLS.

Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa / Baba, M.M., Bitew, M., Fokam, J., Lelo, E.A., Ahidjo, A., Asmamaw, K., Beloumou, G.A., Bulimo, W.D., Buratti, E., Chenwi, C., Dadi, H., D'Agaro, P., De Conti, L., Fainguem, N., Gadzama, G., Maiuri, P., Majanja, J., Meshack, W., Ndjolo, A., Nkenfou, C., et al.. - In: ECLINICALMEDICINE. - ISSN 2589-5370. - 40:(2021), pp. 101101."-"-101101."-". [10.1016/j.eclinm.2021.101101]